精品久久人人妻人人做精品,伊人色啪啪天天综合婷婷,国语自产少妇精品视频蜜桃在线,精品欧美一区二区在线观看

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>Panc 02.03 人胰腺癌細(xì)胞

Panc 02.03 人胰腺癌細(xì)胞

簡要描述:CRL-2553 Panc 02.03 人胰腺癌細(xì)胞,
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號:
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-12
  • 訪  問  量:1678

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-2553 Panc 02.03 人胰腺癌細(xì)胞

ATCC® Number: CRL-2553™ Price: $417.00

Designations: Panc 02.03

Depositors: EM Jaffee

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial

Source: Organ: pancreas

Disease: adenocarcinoma

Cellular Products: cytokeratins 7 and 18 [50655]

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


CRL-2553 Panc 02.03 人胰腺癌細(xì)胞

Tumorigenic: Yes

Oncogene: K-ras +

Antigen Expression: MHC class I +; MHC class II - [50655]

DNA Profile (STR): Amelogenin: X

CSF1PO: 11,12

D13S317: 12

D16S539: 11

D5S818: 12,13

D7S820: 9,10

THO1: 6

TPOX: 9,12

vWA: 17

Age: 70 years

Gender: female

Ethnicity: White

Comments: Panc 02.03 is a pancreatic adenocarcinoma epithelial cell line derived in 1995 from a primary tumor removed from the head-of-the-pancreas of a female with pancreatic adenocarcinoma.

The cell line exhibits a K-ras oncogene mutation at codon 12 where a GGT --> GAT mutation resulted in substitution of aspartic acid for glycine. [50655]

The cells have a reported plating efficiency of 100%. [50655]

Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 10 Units/ml human insulin, 85%; fetal bovine serum, 15%

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 28.2 hrs [50655]

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 50655: Jaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602



CRL-2553

來自ATCC細(xì)胞,所有出入庫細(xì)胞均經(jīng)過嚴(yán)格的細(xì)胞質(zhì)量檢測,確保細(xì)胞無污染,符合檢測合格標(biāo)準(zhǔn)。歡迎來詢價詳談

















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2024 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
色欧美片视频在线观看| 人人妻人人澡人人爽国产| 国产东北露脸熟妇| 熟妇五十路六十路息与子| 亚洲欧洲中文日韩久久AV乱码| 国产精品久久久久AAAA| 沦为公妓的清纯校花H| 国内老熟妇对白XXXXHD| 色噜噜狠狠一区二区三区| 久久久久久无码日韩欧美| 麻豆成人久久精品二区三区| 四虎成人精品在永久免费| 自慰内裤没脱就开始要流水了| 久久久久99精品成人片A片试看 | 国产又黄又爽又色又刺激视频| 风雨送春归电视剧全集在线观看| 国语自产拍精品香蕉在线播放 | 无码精品国产一区二区三区免费 | 亚洲AV无码有乱码在线观看| 成人无遮挡18禁免费视频| 97精品久久久久中文字幕| 妺妺窝人体色777777| 东北熟妇啪啪流白浆| 精品无人区麻豆乱码1区2区| 乱VODAFONEWIFI熟妇| A级A片少妇高潮喷水片| 客厅玩朋友娇妻HD完整版视频| 上司揉捏人妻丰满双乳电影| 无码人妻少妇久久中文字幕| 久久精品国产亚洲AV无码娇色| 8AV国产精品爽爽ⅤA在线观看| FREE性欧美人与DOOG| 天天躁日日躁狠狠躁午夜剧场| 全免费A级毛片免费看表情包| 别揉我奶头~嗯~啊~动漫网站| 爱我久久免费观看高清| 中文字幕爆乳巨爆乳系列| 亚洲色婷婷久久精品AV蜜桃| 我C了语文课代表嗷嗷叫了一节课| 绝伦の上司に一晚人妻 | 精品无码一区二区三区爱欲九九|