精品久久人人妻人人做精品,伊人色啪啪天天综合婷婷,国语自产少妇精品视频蜜桃在线,精品欧美一区二区在线观看

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>SK-OV-3人卵巢癌細(xì)胞

人卵巢癌細(xì)胞

簡要描述:HTB-77 SK-OV-3 人卵巢癌細(xì)胞,
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞,細(xì)胞庫管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件

  • 產(chǎn)品型號:SK-OV-3
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-12
  • 訪  問  量:2192

產(chǎn)品分類

Product Category

詳細(xì)介紹

HTB-77 SK-OV-3 人卵巢癌細(xì)胞

ATCC® Number: HTB-77™ Price: $272.00

Designations: SK-OV-3 [SKOV-3]

Depositors: G Trempe, LJ Old

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial

HTB-77 SK-OV-3 人卵巢癌細(xì)胞

Source: Organ: ovary

Disease: adenocarcinoma

Derived from metastatic site: ascites

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Restrictions: The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; (212) 717-3439.

Isolation: Isolation date: 1973

Applications: transfection host (Nucleofection technology from Lonza

Roche FuGENE® Transfection Reagents)

Tumorigenic: Yes

Antigen Expression: Blood Type B; Rh+

DNA Profile (STR): Amelogenin: X

CSF1PO: 11

D13S317: 8,11

D16S539: 12

D5S818: 11

D7S820: 13,14

THO1: 9,9.3

TPOX: 8,11

vWA: 17,18

Cytogenetic Analysis: This is a hypodiploid human cell line. The modal chromosome number was 43, occurring in 63.3% of cells. The range was 42 to 45. The rate of higher ploidies was 32%. The del(1)(q21), der(13)t(1;?;13) (q11;?;q34), der(11)t(11;?) (q12), del(10)(q22) and 3 other marker chromosomes were common to most cells, and 3 others were found only in some cells. One N11 had the HSR segment from p11 to the distal end. The normal N10, N12, N15, N17 and N19 were absent. Others were either single or paired. There were from 1 to 6 rearranged and unassignable chromosomes. The X chromosome was either single or paired.

Isoenzymes: AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1-2

Me-2, 1

PGM1, 1-2

PGM3, 1

Age: 64 years

Gender: female

Ethnicity: Caucasian

Comments: SK-OV-3 cells are resistant to tumor necrosis factor and to several cytotoxic drugs including diphtheria toxin, cis-platinum and adriamycin.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:6 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2007

recommended serum:ATCC 30-2020

References: 21869: . Human tumor cells in vitro. New York: Plenum Press; 1975.

22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

23103: Morimoto H, et al. Overcoming tumor necrosis factor and drug resistance of human tumor cell lines by combination treatment with anti-Fas antibody and drugs or toxins. Cancer Res. 53: 2591-2596, 1993. PubMed: 7684321

23478: Morimoto H, et al. Synergistic effect of tumor necrosis factor-alpha- and diphtheria toxin- mediated cytotoxicity in sensitive and resistant human ovarian tumor cell lines. J. Immunol. 147: 2609-2616, 1991. PubMed: 1918981

32281: Zhang X, et al. Microfilament depletion and circumvention of multiple drug resistance by sphinxolides. Cancer Res. 57: 3751-3758, 1997. PubMed: 9288783

32456: Clinton GM, et al. Estrogens increase the expression of fibulin-1, an extracellular matrix protein secreted by human ovarian cancer cells. Proc. Natl. Acad. Sci. USA 93: 316-320, 1996. PubMed: 8552629

32530: Zhang X, Smith CD. Microtubule effects of welwistatin, a cyanobacterial indolinone that circumvents multiple drug resistance. Mol. Pharmacol. 49: 288-294, 1996. PubMed: 8632761

90272: Wiechen K, et al. Suppression of the c-erbB-2 gene product decreases transformation abilities but not the proliferation and secretion of proteases of SK-OV-3 ovarian cancer cells. Br. J. Cancer 81: 790-795, 1999. PubMed: 10555747

90273: Yu D, et al. Enhanced c-erbB-2/neu expression in human ovarian cancer cells correlates with more severe malignancy that can be suppressed by E1A. Cancer Res. 53: 891-898, 1993. PubMed: 8094034

90274: Karlan BY, et al. Glucocorticoids stabilize HER-2/neu messenger RNA in human epithelial ovarian carcinoma cells. Gyn. Onc. 53: 70-77, 1994. PubMed: 7909787



HTB-77 SK-OV-3




















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
午夜精品久久久久久| 久久久久黑人强伦姧人妻| 中文在线っと好きだっ| 久久久无码精品亚洲日韩按摩| 伊人激情AV一区二区三区| 好男人好资源在线观看免费视频 | 女生和男生一起差差30分app| 邻居少妇张开双腿让我爽一夜| 一本一本久久A久久综合精品| 日韩99在线 | 中文| 日本亚洲色大成网站WWW久久| 两个领导在车里吃我奶 | 日产一二三区别免费必看| 老师在办公室被躁在线观看 | 麻豆人妻少妇精品无码专区| 蜜桃成人无码区免费视频网站| 短篇乱肉欲文500篇| 被调教的少妇雅芳1一19| 丰满多毛的大隂户视频| 中国帅气体育生GARY视频| 亚洲AV无码乱码精品国产| 邻居的色诱中文字幕| 国产高清在线A视频大全| 亚洲色婷婷久久精品AV蜜桃| 亚洲中文一本无码AV在线观看| 成人欧美一区二区三区在线观看| 美女高潮无遮挡免费视频| 少妇寂寞偷公乱400章深夜书屋 | 亚洲最大成人网| 久久久久亚洲AV成人网人人软件| 边吃奶边狠狠躁日韩A片| 妺妺窝人体色WWW美女| 性色AV浪潮AV色欲AV一区| 狠狠夜色午夜久久综合热| 中文无码日韩欧免费视频APP| 久久精品人妻一区二区蜜桃| 控精止泄教程锻炼法| 人妻 白嫩 蹂躏 惨叫| 国产我和子的与子乱视频| 亚洲熟妇无码另类久久久| 特黄 做受又硬又粗又大视频|